You can align translated sequences by selecting the Translate nucleotide sequence(s) prior to alignment option. When aligning nucleotide sequences, you may also choose to translate the sequence and align the Amino Acid sequences rather than the underlying nucleotides. The Result Name option allows you to choose a custom file name for your new alignment. The Expected Output options allow you to add a tree or customise your alignment algorithm. You may also wish to extract a certain region or translate your nucleotide sequence prior to alignment. You will always be able to produce an alignment of your entire sequences (whether your sequence is nucleotide or protein). When you align sequences you have several options, as shown below. To align sequences directly from the Files table, (1) select more than one sequence, (2) click Post-processing and (3) Align in the dropdown. Adding extra sequence information to an alignment.Aligning from the All Sequences result table.Alignment can be performed using the Align operation under the Post-processing tab. Pairwise and multiple sequence alignment can be performed both before and after sequence annotation. Aligning sequences is a good way of visually comparing sequences, and can include a tree, assay data, and cluster information.
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